IHC Enzyme Antigen Retrieval Reagent

Manufacturer Boster
Type Solution
Specific against other
Format IHC Related Reagent
Applications IHC
Amount 50mL (for 500-750 assays)
Item no. BOS-AR0022
eClass 6.1 32120508
eClass 9.0 32120508
The IHC enzyme antigen retrieval compound digest solution contains multiple proteinases that catalyze proteolysis in tissue samples, breaking the proteins into smaller peptide fractions and amino acids. Enzymatic digestion is commonly used in IHC in the PIER method for antigen retrieval to overcome the lost immunoreactivity of epitopes caused by the covalent cross-links that are formed in tissues during formalin fixation. Proteolysis acts to improve the accessibility of antibodies to target antigens by two main mechanisms: by increasing the penetration of immunoreagents into tissue structures as a result of both tissue digestion and relaxation of the rigid protein structure, and by restoring the methylene-bridged antigenic sites. Since proteases exhibit different cleavage site specificity, the combination of multiple proteinases provides a broader spectrum of proteins digestion in a tissue sample. Thus various types of antigenic targets (e.g. protein, glycoprotein, or non-protein, inter- or intracellular antigens) can benefit from this cocktail of digestion enzymes.
Compound digest solution is a ready to use proteolytic enzyme solution intended to be used as a pretreatment reagent in IHC for enzymatic digestion of tissues employed in proteolytic induced epitope retrieval (PIER) techniques applied upon paraffin-embedded formalin-fixed (PEFF) tissue sections on slides following deparaffinization and prior to antibody staining.
Short description
IHC enzyme antigen retrieval reagent is a ready to use IHC reagent solution containing proteolytic enzymes intended to be used for enzymatic digestion in immunostaining assay procedures, including enzymatic (proteolytic) antigen retrieval in paraffin-embedded, formalin-fixed tissues
Store at 4C. Avoid multiple freeze-thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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Amount: 50mL (for 500-750 assays)
Available: Out of stock
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