Comparison

Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow) European Partner

Item no. BOS-SA2021
Manufacturer Boster
Amount 1 kit (1200 cm2)
Category
Type Detection Kits
Applications WB
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Goat IgG DAB Chromogenic Kit (Yellow)
Similar products Goat IgG DAB Chromogenic Kit (Yellow)
Available
Storage Conditions
?20C for one year. DAB reagent should be protected from light.
Kit Components
1. Blocking buffer: 2x10g protein dry powder.
Make the blocking buffer by dissolving 2 g protein dry powder in 100 ml Diluent buffer.
2. HRP-conjugated rabbit anti-goat IgG: 0.2 ml.
3. DAB chromogenic reagent, containing:
˙ Chromogenic reagent A: DAB concentrated solution, 3ml. 40x.
˙ Chromogenic reagent B: H2O2 concentrated solution, 3ml. 40x.
˙ Chromogenic reagent C: TBS concentrated buffer, 3ml. 40x.
(Volume is sufficient for covering up to 1200 cm2 of membrane.)
Material Required But Not Provided
˙ Nitrocellulose or PVDF membrane.
˙ Diluent Buffer (for preparation of blocking reagent and antibody dilutions): Add 2.42g Tris, 9g NaCl,
850?900ul pure acetic acid into 1000ml distilled water, adjust pH to 7.2?7.6.
˙ Wash Buffer: Add 0.5 ml of TWEEN 20 into 1000 ml of diluent buffer.
˙ Primary antibody: This kit applies to the primary antibodies raised from goat.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 kit (1200 cm2)
Available: Out of stock
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