Manufacturer |
Boster
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Solution |
Amount |
20ug |
Item no. |
BOS-PROTP55854 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Contents |
The SUMO3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl, 1mM DTT and 20% glycerol. |
Description |
SUMO3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 112 amino acids (1-92a.a.) and having a molecular mass of 12.6kDa (molecular weight on SDS-PAGE will appear higher). SUMO3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Gene name |
SUMO3 |
Protein function |
Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 (PubMed:11451954, PubMed:18538659, PubMed:21965678). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744). |
Purification |
Greater than 95% as determined by SDS-PAGE. |
Shipping |
Shipped with wet ice |
Short description |
SUMO3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 112 amino acids (1-92a.a.) and having a molecular mass of 12.6kDa (molecular weight on SDS-PAGE will appear higher). SUMO3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Storage |
Store in -20° C for long term storage. After reconstitution, store in 4° C for short term usage within a few days. Avoid freeze-thaw cycles. |
Subcellular localization |
Cytoplasm. Nucleus. Nucleus, PML body |
Tissue specificity |
Expressed predominantly in liver. |
Format Information |
Sterile Filtered clear solution. |
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