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Anti-Human CD80 - FITC

Anti-Human CD80 - FITC

Item no.	LEIN-C1534-25tests
Manufacturer	Leinco Technologies
Amount	25 Tests
Quantity options	100 Tests 25 Tests
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	FC
Clone	37711
Specific against	Human (Homo sapiens)
Host	Mouse
NCBI	941
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Flow Cytometry: This conjugate has been pre-diluted to use approximately 10 µl per test when staining cells for flow cytometry. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁶ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes and resuspended in 200-400 µl of 1X PBS.
Manufacturer - Category	Primary Monoclonal Antibodies>Immune Checkpoints\|Primary Monoclonal Antibodies>SurfaceTag Human CD Markers
Manufacturer - Targets	CD80
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	This Fluorescein (FITC) conjugate is stable for at least twelve months when stored at 2-8°C protected from light. Do not freeze.
Product Description	CD80 is a highly glycosylated 60 kD protein that is part of the Ig superfamily and is significantly involved in immune cell activation in response to pathogens. CD80 is closely related to, and works in tandem with CD86 (B7-2) to prime T- cells. CD80 binds to CTLA-4 to deliver an inhibitory signal to T cells. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. It is thought that CD80 interacts with a ligand on Natural Killer cells, activating the Natural Killer cell-mediated cell death of the CD80 carrier. The activation of Natural Killer cell-mediated death via CD80 interactions has potential as a possible cancer immunotherapy through the induction of CD80 expression on tumor cells.
Background	CD80 is a highly glycosylated 60 kD protein that is part of the Ig superfamily and is significantly involved in immune cell activation in response to pathogens. CD80 is closely related to, and works in tandem with CD86 (B7-2) to prime T- cells. CD80 binds to CTLA-4 to deliver an inhibitory signal to T cells. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. It is thought that CD80 interacts with a ligand on Natural Killer cells, activating the Natural Killer cell-mediated cell death of the CD80 carrier. The activation of Natural Killer cell-mediated death via CD80 interactions has potential as a possible cancer immunotherapy through the induction of CD80 expression on tumor cells.
PubMed	CD80
Excitation Laser	Blue Laser (488 nm)
Ligand/Receptor	CD28, CD152 (CTLA-4)
Manufacturer - Specificity	Clone 37711 recognizes an epitope on human CD80.
RRID	AB_2828603
Formulation	This monoclonal antibody is conjugated to fluorescein and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 0.1% sodium azide as a preservative.
Antigen Distribution	CD80 is expressed on activated B cells, monocytes/macrophages, and dendritic cells.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C1534-25tests-safety-datasheet.pdf