Comparison

OCAM Antibody

Item no. PRS-45-990-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications IHC, ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Caprine
Conjugate/Tag Unconjugated
Citations Beecken WD, Engl T, Ogbomo H, Relja B, Cinatl J, Bereiter-Hahn J, Oppermann E, Jonas D, Blaheta RA. Valproic acid modulates NCAM polysialylation and polysialyltransferase mRNA expression in human tumor cells. Int Immunopharmacol. 2005 Apr;5(4):757-69.
NCBI NCAM2
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias NCAM2,neural cell adhesion molecule 2,HGNC:7657,MGC51008,NCAM21,OCAM
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Manufacturer - Research Area
Cancer
By Type
Cancer
Modifications
None
Protein Gi #
33519481
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Application Note
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 150 and 28kDa bands in Human and Mouse Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93.0kDa according to NP_004531.2. Both observed bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Frozen section of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles (right side middle, figure 1) and moderate peripheral staining outside the glomeruli (top, figure 1). NCAM2 (red) is stained complementary to VGLUT2 (blue) and NQO1 (green) in figure 2. Recommended concentration: 0.5ug/ml. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Homology
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen
The immunogen for this antibody is: C-KVSNDIIQSKEDDSK
Clonality
Polyclonal
NCBI Official Name
neural cell adhesion molecule 2
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close