GDI2, Recombinant, Human, aa1-445, His-Tag (Rab GDP Dissociation Inhibitor beta Isoform 1, RABGDIB)

Blue Ice

53, 1

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.

Rab GDP dissociation inhibitor beta isoform 1, also known as GDI2, belongs to the GDP dissociation inhibitors (GDIs) family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin.

Source:
Recombinant protein corresponding to aa1-445 from human GDI2, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~53.1kD (468aa)

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.