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Nop5p + Nop58p [34B12]

Nop5p + Nop58p [34B12]

Item no.	20-272-192584
Manufacturer	GENWAY
Amount	0.25 ml
Category	Antibodies Immunoprecipitation Western Blot Immunofluorescence Primary Antibodies
Type	Antibody
Applications	WB, IF, IP
Clone	34B12
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-EC3BA2
Similar products	20-272-192584
Available
Genway ID:	GWB-EC3BA2
Clone:	34B12
Isotype:	IgG
Immunogen:	This was raised against a yeast nucleolar prep and screened by immunocytochemistry and western blotting.
Target:	Nop5p + Nop58p
Localization:	Nuclear (nucleolus)
Purification Note:	Sterile filtered. Storage
Preservative:	10mM Sodium Azide; Constituents: Tissue Culture Supernatant
Application Note:	IF: Use at a dilution of 1/1000 - 1/10000 (yeast cells). IP: Use 2-4 µ l for the amount of lysate derived from 5 OD600 units of yeast culture. WB: Use at a dilution of 1/10000 (ECL). Detects a band of approximately 67 kDa (predicted
Molecular Weight:	57 kDa). Does not immunoprecipitate as well as another Nop5p monoclonal antibody 20-272-192585. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular
Localization:	Nuclear (nucleolus) Nop5p (a. k. a. Nop58p) is localized in the nucleolus and required for pre-18S rRNA processing in S. cerevisiae (baker\' s yeast).
Function:	Required for pre-18S rRNA processing. May bind microtubules.
Subunit:	Interacts with SIK1/NOP56 and NOP1. Interacts with the trimethylguanosine synthase TGS1. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.
Subcellular Location:	Nucleus nucleolus.
Miscellaneous:	Present with 34700 molecules/cell in log phase SD medium.
Similarity:	Belongs to the NOP5/NOP56 family.
Similarity:	Contains 1 Nop domain.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.25 ml

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Delivery expected until 8/28/2025

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