CD61

CD61NCBI

CD61

Platelet plasma membrane

This product recognises a 105kD glycoprotein which is the integrin beta 3 chain. This molecule associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41 and on endothelial cells monocytes platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen fibronectin vWF vitronectin and thrombospondin.

Phosphate buffered saline pH7. 4

Use 10ul of the suggested working dilution to label 106 cells or 100ul whole bloodSuggested

Integrin alpha-V/beta-3 is a receptor for cytotactin fibronectin laminin matrix metalloproteinase-2 osteopontin osteomodulin prothrombin thrombospondin vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin fibrinogen plasminogen prothrombin thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi\' s sarcoma lesions.

Membrane; Single-pass type I membrane protein.

Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. SHC binding also occurs when Tyr-785 alone is phosphorylated.

Position 169 is associated with platelet-specific alloantigen HPA-4 (PEN or YUK). HPA-4A/PEN(A)/YUK(A) has Arg-169 and HPA-4B/PEN(B)/YUK(B) has Gln-169. HPA-4B is involved in neonatal alloimmune thrombocytopenia (NAIT or NATP).

Position 515 is associated with platelet-specific alloantigen CA/TU. CA(-)/TU(-) has Arg-515 and CA(+)/TU(+) has Gln-515. CA(+) is involved in NAIT.

Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls) have detectable amounts of fibrinogen and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.

Contains 1 VWFA domain. 1. Michelson. A. D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons. Leucocyte Typing V. Oxford University Press p 1230-1231. 2. Phillips. D. R. et al. (1991) GPIIb-IIIa: the responsive integrin. 3. Hynes. R. O. (1992) Integrins: versatility. modulation. and signaling in cell adhesion.