Histone H2B

GWB-89CF7E

A synthetic acetylated peptide corresponding to residues surrounding Histone H2B was used as an immunogen.

WB: 1:1 000 - 10 000IHC: 1:250 - 500ICC: 1:50 - 100IP: 1:30. Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin and is made up of four core histone proteins (H2A H2B H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5 12 15 and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5). 1. Braunstein M et al. Mol. Cell. Biol. 16:43492. Workman JL Kingston RE . Annu. Rev. Biochem. 67: 5453. Cheung P. et al. Cell103 2634. Xiao T et al. Mol Cell Bio 25:637-651 2005. 5. Ahn SH et al. Cell Cycle 4:780-783 2005. 6. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487