SMARCA4 (Transcription Activator BRG1,ATP-dependent Helicase SMARCA4,BRG1-associated Factor 190A,BAF190A,Mitotic Growth and Transcription Activator,Protein BRG-1,Protein Brahma Homolog 1,SNF2-beta,SWI/SNF-related Matrix-

Antibodies / Antibodies-Actin Related Proteins

-20°C

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Recombinant protein corresponding to human SMARCA4 fused to GST-tag.

Also known as Protein BRG1 and SNF2-beta, among other aliases, SMARCA4 a transcriptional activator belonging to the SNF2/RAD54 helicase family, and acts as a component of the SWI/SNF ATP-dependent chromatin remodeling complexes family. BRG1 contains 4 domains of interest; a bromo domain, a helicase ATP-binding domain, a helicase C-terminal domain, and a HAS domain. It is a component of several different complexes with distinctive roles such as the CREST-BRG1 complex which regulates the activation of promoters in a calcium-dependent manner, releasing repressor complexes and recruiting activator complexes. It is also a part of the nBAF and npBAF complexes which are involved in the chromatin remodeling of mononucleosomes and nucleosomal arrays. As a component of the SWI/SNF complex, SMARCA4 has a role in tumor suppression and has been shown to regulate cell cycle and differentiation when present in neoplasma.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml
Immunocytochemistry: 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa nuclear extract

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.