Goat anti-IA2 Antibody

Pep ELISA, WB

Goat anti-IA2 Antibody
Principle Name: IA2
Official Symbol: PTPRN
All Name and Symbols: IA-2 ; protein tyrosine phosphatase, receptor type, N ; FLJ16131 ; IA-2/PTP; IA2 ; ICA512 ; R-PTP-N; islet antigen 2 ; islet cell antigen 512 ; islet cell autoantigen 3 ; protein tyrosine phosphatase-like N
Accession Number(s): NP_002837.1
Peptide Sequence: QGEGNIKKNRHPD, is from internal region
Human Gene ID(s): 5798
Non-Human GeneID(s): 19275 (mouse); 116660 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 110kDa band observed in lysates of cell line NIH 3T3 (calculated MW of 106kDa according to Human NP_002837.1 and Mouse NP_033011.2). Recommended concentration: 0.5-1.5ug/ml.
Reference(s): Buzzetti R, Di Pietro S, Giaccari A, Petrone A, Locatelli M, Suraci C, Capizzi M, Arpi ML, Bazzigaluppi E, Dotta F, Bosi E; Non Insulin Requiring Autoimmune Diabetes Study Group. High titer of autoantibodies to GAD identifies a specific phenotype of adult-onset autoimmune diabetes. Diabetes Care. 2007 Apr; 30(4):932-8.PMID: 17392553