Comparison

SRP72 Antibody (Center)

Item no. ABC-AP6916c
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IF, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products SRP72, Signal recognition particle 72 kDa protein, Signal recognition particle subunit SRP72
Available
Primary Accession
O76094
Antigen Type
Synthetic Peptide
Application
IF, IHC-P, FC, WB
Bio Background
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. It binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
Bio References
Utz, P.J., et.al., J. Biol. Chem. 273 (52), 35362-35370 (1998)
Clonality
Polyclonal
Clone/Animal Names
RB21267
Gene ID
6731
Gene Name
SRP72
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Immunofluorescence analysis of SRP72 Antibody (Center) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Type image 1
IF
Dilution image 1
1:10~50
Legend image 2
All lanes : Anti-SRP72 Antibody (Center) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 75 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Formalin-fixed and paraffin-embedded human pancreas with SRP72 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3
IHC-P
Dilution image 3
1:50~100
Legend image 4
Flow cytometric analysis of hela cells using SRP72 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4
FC
Dilution image 4
1:10~50
Isotype
Rabbit Ig
Calculated MW
74606
Antigen Region
119-148
Antigen Source
HUMAN
Target/Specificity
This SRP72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human SRP72.
NCBI Accession
NP_001254651.1; NP_008878.3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close