« Back
Home /
Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Ultra-Low Endotoxin

Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Ultra-Low Endotoxin

Item no.	ICH1044UL-100MG
Manufacturer	IchorBio
Amount	100 mg
Quantity options	100 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies Western Blot
Type	Antibody
Applications	WB, FC, IP, IHC
Clone	53-6.7
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2a Kappa
Purity	>98% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CD8 alpha chain, Lyt-2, Lyt2
Shipping Condition	Cool pack
Available
Specificity	Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.
Manufacturer - Applications	Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen)
Manufacturer - Category	Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Rat IgG2a Kappa
Manufacturer - Targets	CD8a
Shipping Temperature	Blue ice
Storage Conditions	anti-CD8a In Vivo Antibody - Ultra Low Endotoxin (53-6.7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 1%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution	Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.
Application Notes	Immunoprecipitation: Rat Anti-Mouse CD8a (Clone 53-6.7) precipitates 44 kDa and 33 kDa proteins. Each investigator should determine their own optimal working dilution for specific applications.
Background	CD8, a.k.a Lyt-2, Ly-2, or T8, consists of disulfide-linked A and β chains that form the A(CD8a)/β(CD8b) heterodimer and A/A homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 A/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR A/β T cells.
Concentration	1.0 - 5.0 mg/ml
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.
Purification method	This monoclonal antibody was purified using Protein G
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	Mouse thymus or spleen
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus
Related Antibodies	Anti-CD8a In Vivo Antibody - Low Endotoxin [53-6.7] (ICH1044)
Antibodies against the same target (Cat No.)	ICH1045UL-5MG, ICH1045-1MG
Isotype Control	Rat IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [1-1] (ICH2244UL)
Buffer	ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ICH1044UL-100MG-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 100 mg

available

Delivery expected until 9/4/2025

Express Delivery: Save 8 days

Compare

Add to wishlist

Get an offer