Comparison

Total Phosphatidic Acid Assay Kit (Fluorometric)

Item no. MET-5019
Manufacturer Cell Biolabs
Amount 100 assays
Category
Type Assay-Kit
Specific against other
Dry ice Yes
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Shipping Condition Dry ice
Available
Manufacturer - Category
Kit
Shipping Temp
Dry Ice
Storage Temp
Store the entire kit at -80°C. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
Additional Information
Detects total phosphatidic acid content (PA and LPA) in cell lysate samples
Detection sensitivity of approximately 5 uM phosphatidic acid
Phosphatidic Acid standard curve included
LMO Content
None
Product Line
Metabolism Research
Product Classification
Metabolism Research->Lipoprotein Metabolism->Phospholipid Assays
Description
Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.
Detection Method
Fluorometric
UNSPSC
41116100

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 assays
Available: In stock
available

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