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OxiSelect™ Cellular Antioxidant Assay Kit (Green Fluorescence)

OxiSelect™ Cellular Antioxidant Assay Kit (Green Fluorescence)

Manufacturer	Cell Biolabs
Category	Cell Biology Cell Status Oxidative Stress Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Amount	192 assays
Item no.	STA-349
eClass 6.1	32161000
eClass 9.0	32161090
Available
Description	The OxiSelect Cellular Antioxidant Assay Kit Is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured In a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief Incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin Inhibits the formation of free radicals, and thus DCF formation, In a concentration dependent manner.
Formulation	1. Determine the efficacy of an exogenous antioxidant compound within a cellular environment 2. Each kit provides sufficient reagents to perform up to 192 assays, Including standard curve and unknown samples
Shipping Temp	Room Temp
Storage Temp	Upon receipt, store the DCFH-DA Probe (1000X) and Quercetin at -20C. Avoid multiple freeze/thaw cycles. Store the store the remaining components at 4C.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.