Radius™ 48-Well Cell Migration Assay

The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded In the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel Is removed, allowing migratory cells to move across the area and close the gap.

Upon receipt, aliquot and store the Radius Gel Removal Solution and DAPi Fluorescence Stain at -20C (avoid multiple freeze/thaw cycles), and transfer the Fixation Solution to 4C. Store all other kit components at room temperature.