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DAG (Diacylglycerol) Assay Kit

DAG (Diacylglycerol) Assay Kit

Item no.	MET-5028
Manufacturer	Cell Biolabs
Amount	100 assays
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Dry ice	Yes
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Shipping Condition	Dry ice
Available
Manufacturer - Category	Kit
Shipping Temp	Dry Ice
Storage Temp	Store the entire kit at -80°C. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
Additional Information	Suitable for use with cell lysates Detection sensitivity of 4uM DAG DAG standard included NOTE: Each sample replicate requires 2 assays for proper data calculation.
LMO Content	None
Product Line	Metabolism Research
Product Classification	Metabolism Research->Lipoprotein Metabolism->Phospholipid Assays
Description	Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.
Detection Method	Fluorometric
UNSPSC	41116100

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

MET-5028.pdf

MET-5028-datasheet.pdf

MET-5028-safety-datasheet.pdf