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Lecithin Cholesterol Acyltransferase (LCAT) Activity Assay Kit (Fluorometric)

Lecithin Cholesterol Acyltransferase (LCAT) Activity Assay Kit (Fluorometric)

Item no.	STA-615
Manufacturer	Cell Biolabs
CASRN	57-88-5
Amount	100 assays
Category	Food Safety Other Assays & Kits Other Kits Other Parameters
Type	Assay-Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Shipping Condition	Room temperature
Available
Shipping Temp	Room Temperature
Storage Temp	Upon receipt, store the LCAT Fluorometric Substrate and 50X LCAT Inhibitor at -20°C. Avoid multiple freeze/thaws by aliquoting. The LCAT Fluorometric Substrate Is light sensitive and should be maintained In amber tubes. Store the remainder of the kit at 4°C.
Description	Cell Biolabs’ LCAT Activity Assay Kit utilizes a fluorogenic dual-labeled phosphatidycholine as an LCAT substrate. When uncleaved, the fluorophores In the dual-labeled substrate are In a quenched state, upon hydrolysis by LCAT at the sn-2 position, fluorescent monomer chains are produced which can be measured In a fluorescence microplate reader (Ex. 342 nm/Em. 400 nm) (See Assay Principle). The Lecithin:cholesterol acyltransferase (LCAT) Activity Assay Kit Is a simple, fluorometric assay that quantitatively measures LCAT phospholipase activity In plasma, serum, and lysates In a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, Including blanks and unknown samples. Besides LCAT, this assay can also be used to detect other calcium Independent phospholipase activities such as lipoprotein phospholipase A2 (LP-PLA2).
Formulation	1. Quantitatively measures LCAT phospholipase activity In plasma, serum, lysates, as well as detect other calcium Independent phospholipase activities 2. Simple, fluorometric assay that can perform up to 100 assays

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