Comparison

Pure-IP Western Blot Detection Kit

Manufacturer Cell Biolabs
Category
Type Kit
Specific against other
Amount 20 blots
Item no. PRB-5002
eClass 6.1 32161000
eClass 9.0 32161090
Available
Description
  • Reduces background from contaminating heavy and light antibody chains on IP Western blots
  • Suitable for use with primary IgG antibodies from most species
  • Compatible with chemiluminescent and colorimetric detection systems
Overview
Western blot detection of IP proteins typically has high background resulting from the use of the same primary antibody In both the IP and the Western blot. This background Is reduced using the Pure-iPTM Western Blot Detection Kit by using a proprietary HRP Conjugate for detection of the primary antibody. The conjugate Is specific to properly folded antibodies and therefore Is unable to detect the contaminating denatured heavy and light chains present on the membrane.
The Pure-iPTM Western Blot Detection Kit Is compatible with existing protocols for sample preparation, IP, SDS-PAGE and transfer. After transfer, membranes are blocked with the kit Pure-iPTM Blocking Solution and then probed with a primary antibody diluted with Pure-iPTM Blocking Solution. An HRP conjugate Is then used for detection In place of a secondary antibody.
Shipping Temp
Room Temp
Storage Temp
Store the HRP conjugate at -20C. Store the remaining components at 4C.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 blots
Available: In stock
available

Delivery expected until 6/21/2024 

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