Rabbit Anti-Bovine IgG + IgA + IgM HRP pAb

Store undiluted antibody at 2-8C for short term. For extended storage store at -20C.

Antibodies were raised in rabbit to bovine IgG, IgM and IgA. Specific antibodies were absorbed by incubation with Sepharose-bound bovine immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized bovine immunoglobulins a minimum of 65% protein bound. The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtation and ion-exchange chromatography to clear unbound conjugate.