Comparison

Mouse Anti-Human NEFH Clone NF-01 mAb

Item no. CS-CSI14452
Manufacturer Cell Sciences
Amount 25 ug
Category
Type Antibody Monoclonal
Applications WB, IHC
Clone NF-01
Specific against Human (Homo sapiens)
Isotype IgG1
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Similar products NFH, neurofilament triplet H protein, neurofilament, heavy polypeptide 200kDa
Available
Storage Conditions
Upon receipt, store undiluted at 4C C.
Application Notes
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 10 ul per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 10 ug/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Additional reported applications (for the relevant formats) include: immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections, immunocytochemistry.
Description
NF-H is an abundant, stable cytoplasmic protein located in neuronal cells in large axons frequently used as a cell type marker. The NF-H protein shares a high degree of structural and sequence homology with the NF-L and NF-M subunits, especially in the coiled-coil core domain. NF-M and NF-H form flexible extensions linking the neurofilament proteins to each other and other cytoplasmic proteins. Deletions and insertions in the NF-H protein have been reported in amyotrophic lateral sclerosis. The NF-01 monoclonal antibody reacts with a conserved phosphorylated epitope of NF-H present in all species and has been reported to be useful for Western blot, immunohistochemistry using formalin-fixed, paraffin-embedded tissues and immunofluorescence staining.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.
Immunogen
Pellet of pig brain cold stable proteins after microtubule depolymerization
Concentration
0.5 mg/ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 25 ug
Available: In stock
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