Mouse Anti-Human AKT1S1/PRAS40 Clone 73P21 mAb

Human. This antibody does not cross-react with mouse. Other species were not tested.

Purified immunoglobulin in phosphate buffered saline containing 1% BSA. Preservative: 0.1% sodium azide. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.

Purified by Protein A/G affinity chromatography.

Proline-Rich AKT Substrate (PRAS40) is a 40 kDa substrate of AKT. Activated AKT phosphorylates PRAS40 on threonine 246, enabling PRAS40 to bind to 14-3-3. AKT and PRAS40 are components of the phosphoinositide 3-kinase (PI3K) pathway. This pathway plays a role in glucose uptake, cell growth, and apoptosis inhibition. The precise function of PRAS40 is not yet known, however, it has been hypothesized that PRAS40 interacts with SH3 and WW domain containing proteins, and may change the function of these proteins. In Western blot analysis with HeLa cell extracts, this antibody reacts with a 40 kDa band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts.