Anti-Human TNF-R1/TNFRSF1A pAb

For Western Blot use a working dilution of 0.5-4 ig/ml.For Immunohistochemistry use a working dilution of 10-20 ig/ml. The optimal concentration should be determined by the user for each specific application.

Tumor necrosis factor receptor 1 and 2 (TNF-R1 and TNF-R2) are 55 and 75 kDa. While TNF-R1 and TNF-R2 share 28% sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF-R1 contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF-R1 associated death domain protein), and subsequent interactions with FADD. TNF-R1 can also activate the transcription factor NFkB via TRAF2 (TNF receptor associated factor 2). The cytoplasmic domain of TNF-R1 can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade.

Synthetic peptide surrounding amino acid 32 of human TNF-RI

The antibody solution should be gently mixed before use.

0.2 mg/ml