Rabbit Anti-Human APOE pAb

Apolipoprotein E (apoE), a 34 kDa plasma protein and component of LDL.The antigen is isolated from human plasma and used at a purity of 95% as antigen for the immunization of rabbits. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized human albumin and immmunoglobuline fractions in order to remove non-specific antibodies.Specificity of the antisera is confirmed by derect ELISA with purified human apoE adsorbed to plastic wells, by Ouchterlony immunodiffusion, and by dot-blot assay with different fractions of lipoproteins. No interference is caused by delipidated human plasme absorbed by immobilized monoclonal antibody to apoE (MON 5029).