Rabbit Anti-Human APAF1 (NT) Affinity Purified pAb

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf-1 (for apoptosis protease-activating factor 1) (1, 2). Apaf-1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf-1 can also associate with caspase-4 and caspase-8 (4). Apaf-1 is ubiquitously expressed in human tissues (1). This polyclonal antibody can be used for detection of Apaf-1 by Western blot at 1:1000 to 1:2000 dilution. Whole cell lysate from HeLa cells can be used as positive control and a 130 kDa band should be detected. It is human, mouse, and rat reactive. This antibody is for research use only.