Rabbit Anti-Human Bnip3L/Nix (IN) Affinity Purified pAb

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2, 3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1, 2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins. This polyclonal antibody can be used for detection of Bnip3L by Western blot at 0.5 to 1 ug/ml. K562 cell lysate can be used as positive control and the approximate 40 kDa top band represents monomer of Bnip3L (4). It is human and mouse reactive. For research use only.