Rabbit Anti-Human DOK (CT) Affinity Purified pAb

Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories (1, 2). Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP) (1-3). p62dok, represents a new family with very recently identified p56dok (4). This polyclonal antibody can be used for detection of p62dok expression by Western blot at 1:1000 to 1:2000 dilution. Whole cell lysate from Jurkat cells can be used as positive control and a 62 kDa band should be detected. This antibody is for research use only.