Rabbit Anti-Human IKK alpha (C3) Affinity Purified pAb

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The long-sought IkB kinase (IKK), which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb). IKKa and IKKb interact with each other and both are essential for the NF-kB activation. IKKa specifically phosphorylates IkB-a. IKKa is expressed in a variety of human tissues. This polyclonal antibody can be used for detection of IKKa by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate from Jurkat cells can be used as positive control and an 85 kDa band should be detected. It has no cross response to IKKb or IKKg. For research use only.