Rabbit Anti-Human SIRPA(CT) pAb

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2, 3), MyD-1 (4), BIT (5, 6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1, 2, 5, 8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1, 2, 5, 8). This polyclonal antibody can be used for Western blot at 1:1000 to 1:2000 dilution. Whole cell lysate from THP-1 cells can be used as positive control and 75-110 kDa bands should be detected. It is human, mouse, and rat reactive and recognizes SIRPa1, 2 and 3. For research use only.