Rabbit Anti-Porcine Collagen cocktail (types 1, 3) pAb

Antibodies to pig collagen type I and III are raised in rabbits which are numerously immunized with extensively purified native collagen type I and III extracted from pig skin into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction.The affinity purified antibody PS067 is obtained by binding to immobilized native pig collagen, mixture of type I and III (the antigens used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.Specificity was ascertained by direct ELISA using pure pig collagen types I and III for coating microplate wells. No binding to pig serum proteins is revealed at simular dilutions of the antibody. Characteristic immunostaining pictures of frozen sections of pig kidney and skin are produced to certify absence of crossreactivity with basement membrane collagens (type IV and V).