« Back
Home /
Rabbit Anti-Rat PARP1 Affinity Purified pAb

Rabbit Anti-Rat PARP1 Affinity Purified pAb

Manufacturer	Cell Sciences
Category	Oncology Metabolism Cholesterol & Lipid Metabolism Antibodies By Cell Type Carcinoma Hematology Lymphoma Melanoma Sarcoma Cell Biology Cytoskeleton Western Blot Enzymes Prostaglandin Synthases Primary Antibodies small GTPases Arf Subfamily
Type	Antibody Polyclonal
Specific against	Rat
Isotype	IgG
Applications	WB
Amount	100 ug
Host	Rabbit
Item no.	CS-CPP145
Targets	ARFRP1, PARP1
eClass 6.1	32160702
eClass 9.0	32160702
Available
Application Notes	Western blot analysis (1 ug/ml) and immunocytochemistry (10-20, ug/ml). However, the optimal concentrations should be determined individually. The polyclonal anti-cleaved PARP antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP.
Concentration	0.5 mg/ml
Description	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases, in vitro, and is one of the main cleavage targets of caspase-3, in vivo . The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis, in vitro .
Formulation	100 ug (0.2 mg/ml) affinity purified, polyclonal rabbit anti-cleaved PARP antibody in phosphate-buffered saline (PBS) containing 50% glycerol + 1% BSA, and 0.02% sodium azide. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.
Immunogen	Synthetic peptide corresponding to the C-terminus adjacent to Asp214 of mouse PARP
Purification	Affinity purified
Storage and Stability	Store at -20C. For long term storage, aliquot and freeze at -80C. Avoid repeated freeze/defrost cycles.
Specificity	The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CS-CPP145-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 100 ug

available

Delivery expected until 5/23/2024