Mouse Anti-Rat IFN-gamma Clone DB-1 mAb LE/NA

ELISA Capture: The Purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated poly5109 antibody as the detecting antibody and recombinant IFN-a as the standard. Flow Cytometry: The fluorochrome-labeled DB-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-a-producing cells within mixed cell populations.Neutralization: The LE/NA Purified antibody (Endotoxin <0.1 EU/ig, Azide-Free, 0.2 im sterile-filtered) is recommended for neutralization of rat IFN-a bioactivity in vivo and in vitro.Additional reported applications (for the relevant formats) include: Western blotting, and immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections.

Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 um filter sterilized. Endotoxin level is < 0.1 EU/ug of the protein (< 0.01 ng/ug of the protein) as determined by the LAL test.

The LE/NA (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography