Rabbit Anti-Rat Collagen-1 pAb

Store lyophilized product at 4oC. Stable for 1 year from receipt. Reconstituted antibody may be stored at 4C for about 2 months. At 4oC, it is recommended to add an antibicrobial agent such as sodium azide or merthiolate. For longer storage, divide into aliquots, which can be stored at -20C for about 1 year or -80C for several years. Avoid repeated freeze/thaw cycles.

Antibodies to rat collagen type I are raised in rabbits which are numerously immunized with extensively purified native collagen type I extracted from rat tail tendon into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction. Next, the antisera fraction is absorbed with immunobilized rat collagen types III to remove cross-reactive antibodies to antigenic determinants common for this type of collagen. The affinity purified antibody PS065 is obtained by binding to immobilized native rat collagen type I (the antigen used for immunization), followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization. Specificity was ascertained by competition ELISA. Complete inhibition was found if the antibody was pre-incubated with rat collagen type I. Inhibition by rat collagen type III is observed only at 10-20 times higher concentration. No inhibition is found with fibrinectin, fibrogen and laminin. Characteristic immunostaining pictures of frozen sections of rat kidney and skin are produced to certify absence of cross-reactivity with basement membranes.

Native rat collagen type I

Rat collagen type I