Rabbit Anti-Rat Collagen III Affinity Purified pAb

Antibodies to rat collagen type III are raised in rabbits which are numerously immunized with extensively purified native collagen type III extracted from rat tail tendon into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction. Next, the antisera fraction is absorbed with immunobilized rat collagen types I to remove cross-reactive antibodies to antigenic determinants common for this type of collagen. The affinity purified antibody PS066 is obtained by binding to immobilized native rat collagen type III (the antigen used for immunization), followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization. Specificity was ascertained by competition ELISA. Complete inhibition was found if the antibody was preincubated with collagen rat type III. Inhibition by rat collagen type I is observed only at 10-20 times higher concentration. No inhibition was found with fibrinectin. fibrogen and laminin. Characteristic immunostaining pictures of frozen sections of rat kidney and skin are produced to certify absence of crossreactivity with basement membranes.

Reconstitute with 1 ml distilled water and add preservative if preferred.