Human MIP-4/CCL18 ELISA Kit

PARC (Pulmonary and activation-regulated chemokine) belongs to the CC-Chemokines. PARC is referred to also as AMAC-1 (alternative activated macrophage associated CC-Chemokine), MIP-4 (macrophage inflammatory protein-4), or DC-CK1 (dendritic cell-derived chemokine-1). PARC is chemotactic for activated CD3 (+) T-cells and non-activated CD14 (-) lymphocytes, but not for monocytes or granulocytes.The Cell Sciences Human PARC ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PARC in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human PARC coated on a 96-well plate. Standards and samples arepipetted into the wells and PARC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman PARC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PARC bound. The Stop Solutionchanges the color from blue to yellow, and the intensity of the color is measured at 450 nm.