Human Prekallikrein total antigen assay ELISA kit *di

Intended Use: The Human Prekallikrein Total Antigen ELISA Kit is intended for the quantitative determination of Total Prekallikrein Antigen in Human Plasma. Background: Prekallikrein is the glycosylated single chain zymogen precursor of the plasma serine protease Kallikrein. Plasma Prekallikrein circulates with Kininogen and is activated by Factor XIIa in the intrinsic coagulation pathway. Kallikrein activates Plasminogen in fibrinolysis and cleaves Kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time. Elevated plasma Prekallikrein is associated with diabetes and cardiovascular disease. Description: Human Prekallikrein will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, affinity purified polyclonal anti-human prekallikrein primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human prekallikrein. Color development is proportional to the total concentration of prekallikrein in the samples.