Mouse PAI-1 Total ELISA Kit

Intended Use: This assay is intended for the quantitative determination of total Serine (or Cysteine) Peptidase Inhibitor, Clade E, Member 1/Plasminogen Activator Inhibitor-1 (SERPIN E1/PAI-1) in Mouse plasma. Background: PAI-1 is a central regulator of the blood fibrinolytic system. Clinical studies have indicated that increased PAI-1 levels increase the risk for thrombosis, whereas decreased levels may cause recurrent bleeding. Principle of the Assay: Mouse PAI-1 present in plasma reacts with the capture antibody coated and dried on a microtiter plate. Free, latent, and complexed PAI-1 will bind to the plate. Any unbound PAI-1 is washed away and an Anti- PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the total PAI-1 present in the samples, is then reacted with the secondary antibody. Following an additional washing step, TMB is then used for color development at 450 nm. The amount of color development is directly proportional to the concentration of total PAI-1in the sample.