Recombinant Human Apolipoprotein A-I

Lyophilized product is stable at room temperature for up to three weeks. On receipt, store at -20C. After reconstitution, store at 4C for up to one week, or for longer storage keep in working aliquots at -20C. Avoid repeated freeze-thaw cycles.

ApoA-1 is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. ApoA-1, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This apoA-1 ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is thought to inhibit atherogenesis. For this reason HDL is also known as the "good cholesterol." The therapeutic potential of apoA-1 has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-1 (called apoA-1 Milano). The availability of recombinant normal apoA-1 should facilitate further investigation into the potential usefulness of apoA-1 in preventing atherosclerotic vascular diseases. Recombinant human ApoA-1 is a 28.2 kDa protein of 244 amino acid residues

Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers.