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Recombinant Human CD14

Recombinant Human CD14

Manufacturer	Cell Sciences
Category	Neuroscience Neuroinflammation Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human
Amount	10 ug
Host	CHO cells
Item no.	CS-CRCC01
eClass 6.1	34160400
eClass 9.0	42020190
Available
Biological Activity	Up to 20 ug/ml CD14 inhibits binding of FITC-LPS (0.5 ug/ml) to 6 x 105 CD14+ CHO cell transfectants as determined by flow cytometry.
Description	CD14 acts as a receptor for endotoxin (LPS) and is expressed strongly on monocytes, macrophages, and weakly at the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor of LPS-LBP-complexes. Furthermore, CD14 is present in a soluble form in human serum, urine and other bodily fluids, acts at physiological concentrations as an LPS agonist, and has at higher concentrations an antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein. The human CD14 was produced from human CD14 transfected CHO-cells in serum free medium. Before transfection, the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.
Endotoxin Level	<1 EU/ml (LAL test, Charles River)
Formulation	Lyophilized from a concentrated protein solution (0.25 mg/ml) in PBS, pH 7.2.
Molecular Weight	ca.50 kDa
Reconstitution	Centrifuge vial prior to opening. Add 40 ul sterile distilled water to the vial. Further dilutions should be made with PBS or other buffers.
Storage and Stability	The lyophilized product, though stable at room temperature for up to 3 weeks, should be best stored desiccated at -80C for more than 1 year or at 2-8C for about 10 months. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20C. For long term storage it is recommended to add a carrier protein (0.1% BSA). Avoid repeated freeze/thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.