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Recombinant Human CD14 Cell Culture Supernatant

Recombinant Human CD14 Cell Culture Supernatant

Manufacturer	Cell Sciences
Category	Neuroscience Neuroinflammation Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human
Amount	100 ug
Host	CHO cells
Item no.	CS-CRCC02
eClass 6.1	34160400
eClass 9.0	42020190
Available
Biological Activity	50 ug/ml totally inhibits binding of FITC-LPS (0.5 ug/ml) to 6 x 105 CD14+CHO transfectants. Up to 10 ug/ml inhibits binding of LPS-FITC.
Description	Recombinant human CD14 is produced from human CD14 transfected CHO cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The dominant form of the recombinant wild type CD14 is the 50 kDa protein. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. CD14 has been shown to be involved in the LPS-induced cell activation. This antigen is present as a membrane bound form and soluble antigen, both involved in cell stimulation by LPS. The plasma protein LBP plays an important role in this LPS-CD14 mediated cell activation. Monocytes and PMN are activated by the LBP-LPS complex via membrane CD14.
Formulation	Lyophilized from serum free culture supernatant of CHO transfectants (CHO-S-SFM-II + antibiotics, without sodium azide).
Molecular Weight	50 kDa
Reconstitution	Centrifuge vial prior to opening. Add 100 ul sterile distilled water to the vial to fully solubilize the protein to a concentration of 1.0 mg/ml.
Storage and Stability	Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.