Recombinant Human IL-1 alpha

Stable at 2-8C, but best kept desiccated -20C. Upon reconstitution, stable for up to 1 week at 2-8C. For longer term, store in working aliquots below -20C. Avoid repeated freeze/thaw cycles.

IL1 alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL1 alpha and IL1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL1 beta is a secreted cytokine, IL1 alpha is predominantly a cell-associated cytokine.

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Determined by the dose-dependent stimulation of mouse D10S cells. The ED50 is < 0.001 ng/ml.