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Recombinant Human I-309/CCL1

Recombinant Human I-309/CCL1

Manufacturer	Cell Sciences
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human
Amount	10 ug
Host	E.coli
Item no.	CS-CRI400B
eClass 6.1	34160400
eClass 9.0	42020190
Available
Biological Activity	Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using mouse BW5147 T lymphoma cells is less than 9 ng/ml.
Description	Human I-309/CCL1 was initially identified by subtractive hybridization as a transcript that was present in a a/ä T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5f flanking region of their genes.
Endotoxin Level	Less than 1EU/ug as determined by LAL method.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4 + 100 mM NaCl.
Molecular Weight	Approximately 8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.
Reconstitution	Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability	This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
Specificity	>1.1 x 10E5 IU/mg.

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