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Recombinant Human FGF7/KGF-1

Recombinant Human FGF7/KGF-1

Manufacturer	Cell Sciences
Category	Oncology By Cell Type Carcinoma Hematology Myeloma Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton Intermediate filaments
Type	Proteins
Specific against	Human
Amount	10 ug
Host	E.coli
Item no.	CS-CRK300B
Targets	FGF7
eClass 6.1	34160400
eClass 9.0	42020190
Available
Biological Activity	Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 75 ng/ml.
Description	Human KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparin sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51% amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 activates keratinocytes, but exhibits essentially no activity for fibroblasts. KGF-1 has species crossactivity, human KGF-1 shares 96% and 92% amino acid sequence identity with mouse and rat FGF-7, respectively. Recombinant Human KGF-1 is a single, non-glycosylated polypeptide chain containing 163 amino acids.
Endotoxin Level	<1 EU/ug as determined by LAL method.
Formulation	Lyophilized from a 0.2 um sterile filtered solution in 20 mM PB, pH 8.0 + 0.5 M NaCl.
Molecular Weight	ca.18.9 kDa
Reconstitution	Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability	The lyophilized protein is stable at 2-8C. Upon receipt, store desiccated at -20C. After reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.
Specificity	>1.3 x 104 IU/mg.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.