Recombinant Human MIP-5/CCL15

This lyophilized preparation is stable at 2-8C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.

CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (a.a.) protein containing a putative signal peptide of 21 (a.a.) that is cleaved to generate a 92 a.a. residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% a.a. homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1gamma, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Less than 1 EU/ug of rHuMIP-5/CCL15 as determined by LAL method.