Recombinant Human NEK7 GST Active

Recombinant full length human NEK7 containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells. Nek7 is a member of the NIMA (never in mitosis, gene A) family of serine/threonine kinases. In contrast to the other documented NIMA-related kinases, Nek7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies suggest that Nek7 is cytoplasmic and located on chromosome 1. During early embryogenesis Nek7 is expressed in the site of decidual reaction while later in embryogenesis, it is almost exclusively restricted to the nervous system in the dorsal thalamus. The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) Thr412, was purified from rat liver and identified as Nek7. Nek7 kinase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner.

Store product frozen at or below -70C. Stable for 1 year at -70C as undiluted stock. Aliquot to avoid repeated thawing and freezing.