Synthetic Human Thyrotropin Releasing Hormone

Lyophilized protein, although stable at room temperature for 3 weeks, should be stored desiccated at -20C to -80C. Upon reconstitution, the protein may be stored at 2-4C for up to 7 days and for future use it should be aliquoted and frozen at -20C to -80C For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.

Thyrotropin-releasing hormone (TRH), also called thyrotropin-releasing factor (TRF), thyroliberin or protirelin, is a tripeptide hormonethat stimulates the release of thyroid-stimulating hormoneand prolactinby the anterior pituitary. TRH is produced by the hypothalamus, near the paraventricular nucleus. It travels across the median eminence to the pituitary via the hypophyseal portal system. It is released from cells called thyrotropes. In addition to the brain, TRH can also be detected in other areas of the body including the gastrointestinal system and pancreatic islets. Protirelin stimulates the secretion of pituitary thyroid stimulating hormone from the anterior pituitary and has been shown that protirelin increases secretion of prolactin. Protirelin is identified as 5-oxo-L-prolyl-L-histidyl-L-proline amide. It is a synthetic tripeptide that is believed to be structurally identical to the naturally-occurring thyrotropin-releasing hormone produced by the hypothalamus. Thyrotropin Releasing Hormone Human C16H22N6O4 has a MW = 362.39 Dalton.

It is recommended to reconstitute the lyophilized TRH in sterile, distilled water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.