Comparison

Recombinant Human TNF-alpha

Item no. CS-CRT100B
Manufacturer Cell Sciences
Amount 50 ug
Category
Type Proteins
Specific against Human (Homo sapiens)
Host E.coli
Purity >98% by HPLC and SDS-PAGE
Sequence MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products TNF-alpha, TNF, TNF-a, TNFA, TNFSF2, member 2, tumor necrosis factor, TNF superfamily, DADB-70P7.1, DIF, APC1 protein, macrophage-derived, monocyte-derived, cachectin, tumor necrosis factor alpha, tumor necrosis factor ligand superfamily member 2
Available
Manufacturer - Category
Biomolecules
Storage Conditions
The lyophilized protein is stable at 2-8C. Upon receipt, store desiccated at -20C. After reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.
Molecular Weight
ca.17.5 kDa
Description
Tumor necrosis factor alpha (TNF-alpha), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-alpha occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Human and mouse TNF-alpha show approximately 79% homology at the amino acid level and cross reactivity between the two species. Two types of receptors for TNF-alpha have been described and virtually all cell types studied show the presence of one or both of these types. Recombinant Human TNF-alpha is a single, non-glycosylated polypeptide chain containing 158 amino acids.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB +10 mM NaCl, pH 7.0
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Specificity
>2 x 107 IU/mg
Biological Activity
Fully biologically active when compared to the standard. The ED50 as determined by a cytotoxicity assay using mouse L929 cells is < 0.05 ng/ml, corresponding to a specific activity of >2 x 107 IU/mg in the presence of actinomycin D.
Endotoxin Level
<1 EU/ug of TNF alpha as determined by LAL method

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ug
Available: In stock
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