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Recombinant Human TNF-alpha, Endotoxin Free

Recombinant Human TNF-alpha, Endotoxin Free

Item no.	CS-CRT168B
Manufacturer	Cell Sciences
Amount	50 ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Molecular Targets for Neuroscience Aging & Neurological Disorders Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	>98% by SDS-PAGE. Purified product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using custom human Multiplex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Similar products	TNF-alpha, TNF, TNF-a, TNFA, TNFSF2, member 2, tumor necrosis factor, TNF superfamily, DADB-70P7.1, DIF, APC1 protein, macrophage-derived, monocyte-derived, cachectin, tumor necrosis factor alpha, tumor necrosis factor ligand superfamily member 2
Available
Manufacturer - Category	Biomolecules
Storage Conditions	The lyophilized protein, though stable at room temperature for two weeks, is best stored at -20C. Reconstituted protein should be used immediately or stored in working aliquots at -20C. Avoid repeated freeze-thaw cycles .
Molecular Weight	Projected MW = 19.6 kDa. The recombinant protein migrates as two bands with an apparent MW = 21 and 23 kDa in SDS-PAGE.
Description	Recombinant human TNF-alpha protein contains 158 amino acid residues and a 16 aa His-tag. Total length is 174 aa.
Formulation	Lyophilized from a 0.2 um sterile filtered solution of PBS, pH 7.2.
Reconstitution	Centrifuge vial prior to opening . First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.
Biological Activity	The ED50 is determined by the Cytotoxicity Assay for the human- TNF- alpha for dose-dependent cytolytic effects of serial dilutions of h-TNF-alpha on the fibrosarcoma L-929 cells. The ED50 for a typical batch of recombinant human TNF-alpha is 40 ng/ml.
Endotoxin Level	< 0.005 ng per ug of product (0.05 EU/ug) as measured by turbidimetric kinetic assay.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 ug

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Delivery expected until 8/14/2025

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