Recombinant Human Ubiquitin-Conjugating Enzyme E2M His

Store lyophilized protein at -20C to -80C. Reconstituted protein is stable for 1 week at 2-4C. For long term storage, aliquot and store at -20C to -80C with a carrier protein (0.1% HSA or BSA) as a stabilizer. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.

UBE2M is functional in, in vitro, NEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APP-BP1 binds to the amyloid precursor protein (APP) carboxy terminal domain and is important in conjunction with Uba3 and UBE2M in driving cells through the S to M checkpoint. It was demonstrated to be the E2 responsible for the NEDDylation of the Cul-1 component of the SCF(a-TRCP) complex which is important as the E3-ligase in the ubiquitinylation of I B. NEDDylation of Cul-1 is essential for conjugation and processing of NF-eB p105 by SCF(a-TRCP) following phosphorylation of the complex. Recombinant Human Ubiquitin Conjugating Enzyme E2M is a 25 kDa protein containing 216 amino acids including a 6x His tag. It is purified by proprietary chromatographic techniques.

Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.

ca.1mg/ml