Recombinant Mouse IL-6

This lyophilized preparation is stable for several weeks at 2-4C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles

Interleukin-6 (IL-6) is a multifunctional protein that plays important roles in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by a variety of normal and transformed cells including T-cells, B-cells, monocytes/macrophages, fibroblasts, hepatocytes, keratinocytes, astrocytes, vascular endothelial cells, and various tumor cells. The production of IL-6 is upregulated by numerous signals including mitogenic or antigenic stimulation, LPS, calcium ionophore, IL-1, IL-2, IFN, TNF, PDGF, and viruses. IL-6 expression in monocytes is inhibited by IL-4 and IL-13. Recombinant Mouse IL-6 is a single, non-glycosylated polypeptide chain containing 187 amino acids.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <=-20C. Further dilutions should be made in appropriate buffered solutions.

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent mouse 7TD1 cells is <0.02 ng/ml, corresponding to a specific activity of >5 x 107 units/mg.